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Merck
CN

D7690

Monoclonal Anti-DNA Ligase I antibody produced in mouse

~1 mg/mL, clone 1A9, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02686095

Key Documents

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Product Name

Monoclonal Anti-DNA Ligase I antibody produced in mouse, ~1 mg/mL, clone 1A9, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~125 kDa

species reactivity

bovine, human

concentration

~1 mg/mL

technique(s)

direct ELISA: suitable
western blot: 1-5 μg/mL using HeLa or DiFi cells

isotype

IgG1

UniProt accession no.

P18858

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... LIG1(3978)

Application

Monoclonal Anti-DNA Ligase I antibody produced in mouse is suitable for direct ELISA and western blot at a working concentration of 1-5μg/mL using HeLa or DiFi cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DNA ligase I is composed of a 78kDa carboxyl-terminal catalytic domain and a 24kDa amino-terminal region.

Immunogen

bovine DNA ligase I.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

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Storage Class

10 - Combustible liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
065K1187
015K1267
084K1062
044K1203
022K1217

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V J Mackenney et al.
The Journal of biological chemistry, 272(17), 11550-11556 (1997-04-25)
The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is believed to be due to DNA ligase I. This enzyme is composed of a 78-kDa carboxyl-terminal catalytic domain and a 24-kDa amino-terminal region that is not

Articles

DNA Damage and Repair

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

DNA损伤和修复

DNA损伤和修复机制对于维持DNA完整性至关重要。细胞DNA的损伤与突变、癌症发展等有关。

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