D7679
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System
liquid
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.75
form
liquid
Quality Level
technique(s)
immunoblotting: suitable
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
SMILES string
Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key
KJDSORYAHBAGPP-UHFFFAOYSA-N
General description
DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.
Application
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.
Caution
Stable at least one year at 2-8 °C.
Quantity
Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.
Kit Components Only
Product No.
Description
- 10× DAB Liquid Chromogen 10 mL
- premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
WGK
WGK 3
Regulatory Information
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Developmental Biology, 285(2), 477-489 (2005)
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
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For the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (ELISAs)--are the most widely used techniques. Although ELISAs are very sensitive, the obtained sensitivity is not always sufficient. In this study, we have investigated the possibilities
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37(2), 257-263 (1989-02-01)
Immunoblotting techniques are widely used for detection of antigen immobilized on nitrocellulose membranes. There are many immunolabeling methods and staining methods available to disclose the presence of antigen in such techniques. Five common staining methods each for alkaline phosphatase and
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