D7679
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System
liquid
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About This Item
CAS Number:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:
Product Name
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System, liquid
InChI key
KJDSORYAHBAGPP-UHFFFAOYSA-N
InChI
1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
SMILES string
Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
form
liquid
technique(s)
immunoblotting: suitable
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.
Disclaimer
Stable at least one year at 2-8 °C.
General description
DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.
Preparation Note
Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.
Storage Class
11 - Combustible Solids
wgk
WGK 3
Regulatory Information
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37(2), 257-263 (1989-02-01)
Immunoblotting techniques are widely used for detection of antigen immobilized on nitrocellulose membranes. There are many immunolabeling methods and staining methods available to disclose the presence of antigen in such techniques. Five common staining methods each for alkaline phosphatase and
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
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