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D5751

Sigma-Aldrich

Blue Dextran

Void volume marker for gel filtration columns.

Synonym(s):

Dextran blue from Leuconostoc mesenteroides

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About This Item

CAS Number:
87915-38-6
MDL number:
MFCD00130661
UNSPSC Code:
12352200
NACRES:
NA.25

biological source

non-animal source

Quality Level

200

form

powder

quality

200

mol wt

2,000,000

technique(s)

affinity chromatography: suitable

suitability

suitable for GFC marker

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General description

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Application

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Preparation Note

Prepared from dextran with an average mol. wt. of 2,000,000.

Analysis Note

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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