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D5652

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

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Synonym(s):
DPBS
MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

pH

7.2-7.8

solubility

water: 9.6 g/L, clear

storage temp.

2-8°C

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General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Application

D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
  • to dissolve Angelica gigas powder for microencapsulation
  • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
  • to wash amniotic membrane for cellular analyses

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.

  6. Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use.  It can be autoclaved at 121°C, 15 psi for 20 minutes.

  7. Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?

    Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.

  8. What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?

    This product contains 8.0 g/L sodium chloride.

  9. What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?

    The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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