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D5444

Sigma-Aldrich

Anti-DCP1A (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DCP1 Decapping enzyme 1, homolog A, Anti-SMAD4IP1, Anti-SMIF, Anti-Smad 4-interacting transcription factor, Anti-Smad 4-interacting transcriptional co-activator

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

mouse, human, rat (predicted)

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using cell lystes of HEK-293T
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells
western blot: 1-2 μg/mL using cell lysates of HEK-293T

UniProt accession no.

Q9NPI6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DCP1A(55802)
mouse ... Dcp1a(75901)
rat ... Dcp1a(361109)

General description

Dcp1 colocalizes with Dcp2 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. Anti-DCP1A (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at C-terminal of human DCP1A conjugated to KLH. Two distinct genes of human DCP1 were identified, DCP1A and DCP1B, which share ~70% homology in their N-terminal and ~30% homology in their full length.

Application

Anti-DCP1A antibody produced in rabbit is suitable for immunoprecipitation at a working concentration of 5-10 μg using cell lystes of HEK-293T, indirect immunofluorescence at 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells and western blot analysis at 1-2 μg/mL working concentration using cell lysates of HEK-293T. It was used as a primary antibody at a working dilution of 1:200 in the immunofluorescence experiment of HeLa cells treated with 5-fluorouracil to study the assembly of stress granules based on RNA incorporation.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence-cell culture cells (1 paper)

Biochem/physiol Actions

Dcp1 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp2 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

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5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Kaehler C, Isensee J, Hucho T, et al.
Nucleic Acids Research, 42(10), 6436-6447 (2014)
Jianan Liu et al.
Biochemical and biophysical research communications, 515(3), 403-409 (2019-06-04)
Dengue virus (DENV) infection is a public health problem worldwide. To establish infection in host cells, DENV require host cellular mechanism to suppress and evade innate immunity for their replication. In this study, Ccr4-Not complex genes were screened by using
Nadia G D'Lima et al.
Nature chemical biology, 13(2), 174-180 (2016-12-06)
Proteomic detection of non-annotated microproteins indicates the translation of hundreds of small open reading frames (smORFs) in human cells, but whether these microproteins are functional or not is unknown. Here, we report the discovery and characterization of a 7-kDa human
Thomas C Custer et al.
Protein science : a publication of the Protein Society, 26(7), 1363-1379 (2016-12-29)
RNA plays a fundamental, ubiquitous role as either substrate or functional component of many large cellular complexes-"molecular machines"-used to maintain and control the readout of genetic information, a functional landscape that we are only beginning to understand. The cellular mechanisms
Xinmei Wen et al.
Neuron, 84(6), 1213-1225 (2014-12-19)
Expanded GGGGCC (G4C2) nucleotide repeats within the C9ORF72 gene are the most common genetic mutation associated with both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Sense and antisense transcripts of these expansions are translated to form five dipeptide repeat
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