D5319
Deoxyribonuclease I bovine
recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
Synonym(s):
DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
Product Name
Deoxyribonuclease I bovine, recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
biological source
bovine
recombinant
expressed in Pichia pastoris
assay
≥95%
form
buffered aqueous glycerol solution
specific activity
≥5,000 units/mg protein
mol wt
~39 kDa
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
foreign activity
RNAse and protease, free
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.
Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.
Used for the removal of DNA from protein samples.
Biochem/physiol Actions
DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Features and Benefits
- RNA purification by removing DNA
- Prepare DNA for nick translation1
- Footprinting assays to determine DNA-protein interactions2
Other Notes
One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
Physical form
Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
Preparation Note
Produced without using any animal cells or animal derived materials.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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