D5167
Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse
clone 70.1, ascites fluid
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Synonym(s):
Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
70.1, monoclonal
contains
15 mM sodium azide
species reactivity
marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:2,000 using a chick brain extract
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DNAI1(27019) , DNAI2(64446)
rat ... Dnai2(360654)
Related Categories
General description
Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).
Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.
Immunogen
cytoplasmic dynein from chicken embryo brain
Application
Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:
- perturbation assays
- western blotting
- immunofluorescence microscopy
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunocytochemistry
- immunopurification
Biochem/physiol Actions
Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing
Nurminsky DI, et al.
Molecular and Cellular Biology, 18(11), 6816-6825 (1998)
Enzymes for microtubule-dependent motility.
McIntosh JR and Porter ME
The Journal of Biological Chemistry, 264(11), 6001-6004 (1989)
Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
Casenghi M, et al.
Journal of Cell Science, 118(21), 5101-5108 (2005)
Jonne A Raaijmakers et al.
The EMBO journal, 31(21), 4179-4190 (2012-10-05)
The microtubule motor protein kinesin-5 (Eg5) provides an outward force on centrosomes, which drives bipolar spindle assembly. Acute inhibition of Eg5 blocks centrosome separation and causes mitotic arrest in human cells, making Eg5 an attractive target for anti-cancer therapy. Using
Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis
Nakano H, et al.
The Journal of Biological Chemistry, 285(14), 10841-10849 (2010)
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