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D5038

Sigma-Aldrich

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution

100 mM (pH 7)

Synonym(s):

dGTP-Na3

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1 ML
CN¥846.49
10 ML
CN¥4,233.78
25 ML
CN¥5,406.04
100 ML
CN¥17,320.49
500 ML
CN¥70,141.54

CN¥846.49

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1 ML
CN¥846.49
10 ML
CN¥4,233.78
25 ML
CN¥5,406.04
100 ML
CN¥17,320.49
500 ML
CN¥70,141.54

About This Item

Empirical Formula (Hill Notation):
C10H13N5Na3O13P3
CAS Number:
93919-41-6
Molecular Weight:
573.13
EC Number:
300-026-5
MDL number:
MFCD00080337
UNSPSC Code:
41106305
PubChem Substance ID:
24893919
NACRES:
NA.52

CN¥846.49

Please contact Customer Service for Availability

Assay

≥99%

Quality Level

200

form

liquid

concentration

100 mM (pH 7)

color

colorless

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1

InChI

1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1

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HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

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capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

protein purification: suitable

technique(s)

-

form

suspension

form

(1:1 suspension in a 20% ethanol solution)

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.
2′-Deoxyguanosine 5′-triphosphate trisodium salt solution has been used:

  • in a reverse transcription-polymerase chain reaction (RT-PCR) for DNA amplification.[1]
  • as a component of the PCR mix for in situ reverse transcription-polymerase chain reaction (RT-PCR) amplification of mRNA from prokaryotic and eukaryotic ribosomes[2]
  • to study the mutations in the HBB gene via amplification refractory mutation system (ARMS)-PCR[3]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000254651
    0000254651
    0000381275
    0000358746
    0000339470

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    PARP inhibitors (PARPi) cause synthetic lethality in BRCA-deficient tumors. Whether specific vulnerabilities to PARPi exist beyond BRCA mutations and related defects in homology-directed repair (HDR) is not well understood. Here, we identify the ubiquitin E3 ligase TRIP12 as negative regulator

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