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D4913

2′-Deoxycytidine 5′-triphosphate disodium salt

Name: 2′-Deoxycytidine 5′-triphosphate disodium salt
Brand: SIGMA

100 mM (pH 7)

Synonym(s):

2′-Deoxycytidine-5′-triphosphoric acid disodium salt, dCTP-Na₂

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About This Item

Empirical Formula (Hill Notation):

C₉H₁₄N₃Na₂O₁₃P₃

CAS Number:

102783-51-7

Molecular Weight:

511.12

MDL number:

MFCD00084683

UNSPSC Code:

41106305

PubChem Substance ID:

24893907

NACRES:

NA.52

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D7045

2′-Deoxycytidine 5′-triphosphate disodium salt

biological source

synthetic (organic)

Quality Level

200

Assay

≥99%

form

liquid

concentration

100 mM (pH 7)

color

colorless

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2

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PCR & Amplification

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Phosphates

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is a cytidine analog.[1] It is composed of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dCTP is utilized by cells for DNA polymerase catalyzed synthesis of DNA.[2]

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:

in reverse transcription polymerase chain reaction (RT -PCR).[3]
to study the mutations in HBB gene via amplification refractory mutation system (ARMS)-PCR[4]
to C-tail the end-repaired DNA[5]

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sambrook, J., et al.

Molecular Cloning: A Laboratory Manual, C-C (1989)

Structural insights into the recognition of nucleoside reverse transcriptase inhibitors by HIV-1 reverse transcriptase: First crystal structures with reverse transcriptase and the active triphosphate forms of lamivudine and emtricitabine.

Nicole Bertoletti et al.

Protein science : a publication of the Protein Society, 28(9), 1664-1675 (2019-07-14)

The retrovirus HIV-1 has been a major health issue since its discovery in the early 80s. In 2017, over 37 million people were infected with HIV-1, of which 1.8 million were new infections that year. Currently, the most successful treatment

Selection of recombinant antibodies by eukaryotic ribosome display.

Mingyue He et al.

Methods in molecular biology (Clifton, N.J.), 484, 193-205 (2008-07-02)

Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody-ribosome-mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by

Analysis of beta globin gene mutations in Diyarbakir

Tekecs S, et al.

Turkish Journal of Biology (2021)

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.

Michael G Mohsen et al.

Nucleic acids research, 47(18), 9495-9501 (2019-09-11)

We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the

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Key Documents

2′-Deoxycytidine 5′-triphosphate disodium salt

100 mM (pH 7)

About This Item

Recommended Products

Properties

biological source

Quality Level

Assay

form

concentration

color

foreign activity

shipped in

storage temp.

SMILES string

Related Categories

Description

General description

Application

Safety Information

Storage Class Code

WGK

Flash Point(F)

Flash Point(C)

Personal Protective Equipment

Documentation

Certificates of Analysis (COA)

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