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D4818

Sigma-Aldrich

Dispase® I

protease

Synonym(s):

Protease from Bacillus polymyxa

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About This Item

CAS Number:
42613-33-2
EC Number:
255-914-4
MDL number:
MFCD02253017
UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

200

form

lyophilized solid

specific activity

≥10 unit/mg solid

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

storage temp.

2-8°C

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Application

Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.
Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice. The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing. It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Dispase I is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. It can also separate intact epithelial sheets in culture from the substratum. The enzyme preserves the viability of the epithelial cells while cleaving the basement membrane zone region. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues. It preferentially attacks denatured and intercellular proteins with exposed hydrophobic amino acid residues. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity. The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate

Legal Information

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. At what concentration should Product D4818, Dispase, be used?

    To dissociate cells from ECM (extracellular matrix) use at a concentration of 0.6 - 2.4 units/ml.

  4. What is the activity of the Product D4818, Dispase, which I received?

    Each lot will have a specific activity that can be found on our website on the certificate of analysis.

  5. What is the difference between Product D4693, Dispase II  and Product D4818, Dispase I?

    Product D4693 is a lyophilized powder containing calcium acetate and milk sugar as a stabilizer. The activity specification is >0.5 unit/mg solid. Product D4818 does not contain any milk sugar and has an activity specification of >10 units/mg solid.

  6. What is the solubility of Product D4818, Dispase I?

    The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Alizée Vercauteren Drubbel et al.
Cell stem cell, 28(8), 1411-1427 (2021-04-22)
Columnar metaplasia of the esophagus is the main risk factor for esophageal adenocarcinoma. There is a lack of evidence to demonstrate that esophageal progenitors can be the source of columnar metaplasia. In this study, using transgenic mouse models, lineage tracing
Yaojiong Wu et al.
Stem cells (Dayton, Ohio), 25(10), 2648-2659 (2007-07-07)
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed
Liwen Chen et al.
PloS one, 4(9), e7119-e7119 (2009-09-23)
Studies have shown that allogeneic (allo-) bone marrow derived mesenchymal stem cells (BM-MSCs) may enhance tissue repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced engraftment. In this study, we compared allo-BM-MSCs with syngeneic
Some properties of a protease from Bacillus polymyxa.
P J Griffin et al.
The Biochemical journal, 125(4), 109P-109P (1971-12-01)
Marco Tatullo et al.
Materials (Basel, Switzerland), 12(4) (2019-02-20)
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting
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