D4642
Deoxyribonucleic acid from human placenta
Genomic
Synonym(s):
DNA
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About This Item
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grade
for molecular biology
mol wt
10 MDa (≥ 16 kb)
storage temp.
2-8°C
InChI
1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
InChI key
AWBASQCACWFTGD-UHFFFAOYSA-N
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Specificity
42% GC; Tm = 87 °C
Application
For use in Southern hybridizations.
Preparation Note
Purified by equilibrium buoyant density ultracentrifugation in cesium chloride, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
The DNA quality and integrity are evaluated using pulsed field gel electrophoresis and visualization after ethidium bromide staining. The DNA is free of RNA and contaminants that might interfere with restriction endonuclease digestion.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Emilia Huerta-Sánchez et al.
Nature, 512(7513), 194-197 (2014-07-22)
As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to
Sriram Kosuri et al.
Nature methods, 11(5), 499-507 (2014-05-02)
For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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