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D3939

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

peroxidase substrate, liquid, suitable for Immunohistology

Synonym(s):

DAB liquid substrate system

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About This Item

MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24893801
NACRES:
NA.83

Product Name

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride, for Immunohistology

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase.

Application

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used:
  • in the detection of hydrogen peroxide in rice leaves
  • as a substrate for rabbit peroxidase in immunohistochemistry of mice brain sections
  • in immunocytochemistry of muscle-derived stem cells (MDSCs)

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Caution

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Quantity

Each DAB Two-component Liquid Substrate System will provide 100 mL of the DAB substrate solution.

Kit Components Only

Product No.
Description
  • 3, 3′-Diaminobenzidine enhanced liquid substrate system, solution B, for immuno-histochemistry 100 mL

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK8874
SLCM8573
SLCJ2421
SLCH7427
SLCF9150

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