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D3939

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

for Immunohistology

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Synonym(s):
DAB liquid substrate system
MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24893801
NACRES:
NA.83

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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General description

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase.

Application

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used:
  • in the detection of hydrogen peroxide in rice leaves
  • as a substrate for rabbit peroxidase in immunohistochemistry of mice brain sections
  • in immunocytochemistry of muscle-derived stem cells (MDSCs)

The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Caution

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Quantity

Each DAB Two-component Liquid Substrate System will provide 100 mL of the DAB substrate solution.

Kit Components Only

Product No.
Description
  • 3, 3′-Diaminobenzidine enhanced liquid substrate system, solution B, for immuno-histochemistry 100 mL

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of salinity tolerance in rice by constitutive-overexpression of genes involved in the regulation of programmed cell death
Hoang TML
Frontiers in Plant Science, 6, 175-175 (2015)
Chuangao Xie et al.
Oncology letters, 16(1), 940-948 (2018-07-03)
The purpose of the present study was to elucidate the effects of cyclooxygenase 2 (COX-2) on the expression of vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2) in pancreatic cancer in vitro and in vivo, and to clarify the
The neuroprotective effects of muscle-derived stem cells via brain-derived neurotrophic factor in spinal cord injury model
Han D, et al.
BioMed Research International, 2017 (2017)
Mingzhu Yin et al.
Cell reports, 27(9), 2709-2724 (2019-05-30)
The regenerative capacity of the human endometrium requires a population of local stem cells. However, the phenotypes, locations, and origin of these cells are still unknown. In a mouse menstruation model, uterine stromal SM22α+-derived CD34+KLF4+ stem cells are activated and
Łukasz Majewski et al.
Biochimica et biophysica acta, 1864(6), 1071-1087 (2016-12-04)
STIM1 is an endoplasmic reticulum calcium sensor that is involved in several processes in neurons, including store-operated calcium entry. STIM1 also inhibits voltage-gated calcium channels, such as Ca
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