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D3937

Sigma-Aldrich

DirectLoad™ 1 kb DNA Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):

1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker

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Select a Size

10 MG
¥650.36
25 MG
¥1,394.93
100 MG
¥2,699.25
500 MG
¥13,441.78

¥650.36

Available to ship on2025年4月17日Details

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10 MG
¥650.36
25 MG
¥1,394.93
100 MG
¥2,699.25
500 MG
¥13,441.78

About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

¥650.36

Available to ship on2025年4月17日Details

Quality Level

200

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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L4524

Lipopolysaccharides from Escherichia coli O55:B5

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L2637

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5 γ-irradiated, BioXtra, suitable for cell culture

L6529

Lipopolysaccharides from Escherichia coli O55:B5

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

solubility

water: soluble

solubility

water: soluble

solubility

water: soluble

solubility

H2O: 5 mg/mL, faintly hazy to hazy

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.

Application

Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Other Notes

Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ6150
    SLCQ6150
    0000364619
    0000364619
    SLCN2919

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    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

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    Aron M Geurts et al.
    Methods in molecular biology (Clifton, N.J.), 597, 211-225 (2009-12-17)
    The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat

    Articles

    Gel Electrophoresis Markers

    Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

    Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

    Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

    Protocols

    End Point PCR Protocol for Long and Accurate DNA Amplification

    Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

    End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

    Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

    Antibody-Enzyme Mediated Hot Start PCR Protocol

    JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service