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D2915

Sigma-Aldrich

Dexamethasone-Water Soluble

suitable for cell culture, BioReagent

Synonym(s):

Dexamethasone – Cyclodextrin complex

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

product line

BioReagent

form

solid

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 25 mg/mL, clear to slightly hazy

shipped in

ambient

storage temp.

room temp

SMILES string

C[C@@H]1CC2C3CCC4=CC(=O)C=C[C@]4(C)C3[C@@H](O)C[C@]2(C)[C@H]1C(=O)CO.OC[C@H]5O[C@@H]6O[C@H]7[C@H](O)[C@@H](O)[C@H](O[C@@H]7CO)O[C@H]8[C@H](O)[C@@H](O)[C@H](O[C@@H]8CO)O[C@H]9[C@H](O)[C@@H](O)[C@H](O[C@@H]9CO)O[C@H]%10[C@H](O)[C@@H](O)[C@H](O[C@@H]%10CO)O[C@H]%11[C@H](O)[C@@H](O)[C@H](O[C@@H]%11CO)O[C@H]%12[C@H](O)[C@@H](O)[C@H](O[C@@H]%12CO)O[C@H]5[C@H](O)[C@H]6O

InChI

1S/C42H70O35.C22H30O4/c43-1-8-29-15(50)22(57)36(64-8)72-30-9(2-44)66-38(24(59)17(30)52)74-32-11(4-46)68-40(26(61)19(32)54)76-34-13(6-48)70-42(28(63)21(34)56)77-35-14(7-49)69-41(27(62)20(35)55)75-33-12(5-47)67-39(25(60)18(33)53)73-31-10(3-45)65-37(71-29)23(58)16(31)51;1-12-8-16-15-5-4-13-9-14(24)6-7-21(13,2)20(15)17(25)10-22(16,3)19(12)18(26)11-23/h8-63H,1-7H2;6-7,9,12,15-17,19-20,23,25H,4-5,8,10-11H2,1-3H3/t8-,9-,10-,11-,12-,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23-,24-,25-,26-,27-,28-,29-,30-,31-,32-,33-,34-,35-,36-,37-,38-,39-,40-,41-,42-;12-,15+,16?,17+,19-,20-,21+,22+/m11/s1

InChI key

XEJNDGTUKUMQNG-ARJMFYCQSA-N

Related Categories

Application

Dexamethasone-Water Soluble has been used as a component of
  • Dulbecco′s modified Eagle medium based osteogenic and adipogenic medium for human mesenchymal stem cell culture
  • erythroid expansion media for the growth of liver, bone marrow and spleen tissues
  • medium 199 (M199) for hepatocyte culture

Biochem/physiol Actions

Dexamethasone is an anti-inflammatory glucocorticoid and a synthetic corticosteroid used for treating corneal inflammation. It inhibits the IL-1β-dependent angiogenesis. Dexamethasone elicits a range of effects on cell survival, cell signaling and gene expression. It favors apoptosis in chondrocytes and impairs development of bone. Dexamethasone is a potential therapeutic agent as it induces apoptosis in multiple myeloma (MM) cells. It elicits its functionality by lowering glucocorticoid receptor (GR) α levels and inhibits colon tumor progression.

Packaging

Package size based on dexamethasone

Other Notes

Contains approx. 65 mg dexamethasone per gram; balance methyl-β-cyclodextrin.

related product

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. What cell lines has Product D4902, Dexamethasone, been tested on?

    The cell lines on which this product is tested can vary from lot to lot. Some typically used cell lines are 3T3 fibroblasts, BHK, L929 and CHO.

  2. Are Products D4902 and D2915, sterile?

    No. This product is not sterile, but cell culture testing did not find the product to be toxic to cells.

  3. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  4. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  5. How long is Product D2915, Dexamethasone-Water Soluble, stable in solution?

    Dexamethasone solutions are stable for 6 months when aliquoted stored at -20 °C in a non-defrosting freezer and protected from light.  However, insufficient information is available to assess the shelf-life of solutions stored at 4°C.

  6. What is the molecular weight of Product D2915, Dexamethasone-Water Soluble?

    The molecular weight of dexamethasone alone is 392.5 g/mol. Be sure to take the weight of the carrier into account for any calculations involving molarity of solutions.

  7. How much Product D2915, Dexamethasone-Water Soluble, have I received?

    Each vial contains 100mg of Dexamethasone.  The milligrams of Dexamethasone per gram (Dexamethasone content) is listed on the Certificate of Analysis, which can be accessed through our Advanced Search Option.This information can be used to determine the conversion factor to make a solution of a given molarity.

  8. Can I use Product D2915, Dexamethasone-Water Soluble, to inject into mice?

    We do not perform animal studies with our products, but it can be used in cell culture.

  9. How should I reconstitute Product D2915, Dexamethasone-Water Soluble, for tissue culture use?

    The tissue culture grade product is tested in water at a concentration of 25 mg/mL forming a clear to slightly hazy colorless solution.

  10. Do you have references for the use of Product D2915, Dexamethason - Water Soluble?

    Here is one reference that describes using Product No. D2915: Chang, C.-J., et al., Coactivator TIF1? Interacts with Transcription Factor C/EBP? and Glucocorticoid Receptor To Induce ?1-Acid Glycoprotein Gene Expression. Mol. Cell. Biol., 18(10), 5880-5887 (1998).

  11. What is (2-Hydroxypropyl)-β-cyclodextrin contained in Product D2915?

    (2-Hydroxypropyl)-β-cyclodextrin is commonly used to get lipophilic drugs into aqueous solutions. This guest-host type complex is formed between the drug and the non-polar cavity in the HBC that results in enhanced solubility.

  12. Is the (2-Hydroxypropyl)-β-cyclodextrin contained in Product D2915, Dexammethasone - Water Soluble, toxic to cells?

    The carrier, (2-Hydroxypropyl)-β-cyclodextrin, has been found to be non-toxic to mice and rabbits.

  13. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  14. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  15. Can Product D2915, Dexamethasone - Water Soluble, be filter sterilized?

    This product can be filter sterilized through a 0.22 micron filter prior to use.

  16. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mechanisms of dexamethasone-mediated inhibition of Toll-like receptor signaling induced by Neisseria meningitidis and Streptococcus pneumoniae
Mogensen TH, et al.
Infection and Immunity, 76(1), 189-197 (2008)
The effect of temperature on the viability of human mesenchymal stem cells
Reissis Y, et al.
Stem Cell Research & Therapy, 4(6), 139-139 (2013)
Tissue-specific actions of glucocorticoids on apoptosis: a double-edged sword
Gruver-Yates A and Cidlowski J
Cells, 2(2), 202-223 (2013)
Dexamethasone-induced apoptotic mechanisms in myeloma cells investigated by analysis of mutant glucocorticoid receptors
Sharma S and Lichtenstein A
Blood, 112(4), 1338-1345 (2008)
Immature erythroblasts with extensive ex vivo self-renewal capacity emerge from the early mammalian fetus
England SJ, et al.
Blood, 117(9), 2708-2717 (2011)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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