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Merck
CN

D2902

DMEM - low glucose

With ʟ-glutamine and sodium pyruvate, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
Technical Service
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Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 10 g/L, clear

components

glucose: low
NaHCO3: no
phenol red: no
HEPES: no
L-glutamine: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium - low glucose is used in cell line culture (used in medium for the growth of cells) and for their maintenance. It is also used for tissue culture.

Preparation Note

Formulated to contain 10.0 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.


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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids



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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。


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P C Zamecnik et al.
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The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3'- and 5'-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted.
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Lithium has been used as a mood stabilizer to treat human bipolar disorders for over half a century. Several studies have suggested the possibility that the efficacy of lithium treatment results in part from the amelioration of circadian dysfunction. However



Global Trade Item Number

SKUGTIN
D2902-10L04061833562659
D2902-10X1L04061835514052
D2902-25KG04061834408284
D2902-25K04061834180203
D2902-50K04061834180210
D2902-50KG04061834408291
D2902-5K04061834180227