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Key Documents

D1283

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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100 G
CN¥1,118.44

CN¥1,118.44


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100 G
CN¥1,118.44

About This Item

MDL number:
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

CN¥1,118.44


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

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This Item
32318M3231871188
Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

solid

form

crystalline

form

crystalline

form

powder or crystals

storage temp.

2-30°C

storage temp.

-

storage temp.

-

storage temp.

-

pH

7.5-9.2 (20 °C, 50 g/L in H2O)

pH

7.5-9.0 (20 °C, 5%)

pH

7.5-9.0 (20 °C, 5%)

pH

8.5-10.0 (25 °C, 3 M in H2O)

density

1.42 g/cm3 at 20 °C

density

-

density

-

density

-

General description

Dulbecco′s Phosphate Buffered Saline is an isotonic saline solution used for washing cells and tissues. This balanced salt solution (BSS) is also used in cell culture as a buffering system to maintain the medium within the physiological pH range and as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • for decellularization of extracellular matrix (ECM)
  • to rinse biosensor chip to remove physically adsorbed mycolic acids
  • for washing cells before staining during in vitro biocompatibility test

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    Sarah S Gabriel et al.
    Kidney international, 91(4), 842-855 (2017-02-02)
    Dent disease is a rare X-linked tubulopathy caused by mutations in the endosomal chloride-proton exchanger (ClC-5) resulting in defective receptor-mediated endocytosis and severe proximal tubule dysfunction. Bone marrow transplantation has recently been shown to preserve kidney function in cystinosis, a
    Nasim Shahidi Hamedani et al.
    Journal of clinical medicine, 10(4) (2021-02-14)
    Coagulation factor XIII (FXIII) is a protransglutaminase which plays an important role in clot stabilization and composition by cross-linking the α- and γ-chains of fibrin and increasing the resistance of the clot to mechanical and proteolytic challenges. In this study
    Alba Di Pardo et al.
    Cell death & disease, 11(7), 546-546 (2020-07-20)
    The longevity-associated variant (LAV) of the bactericidal/permeability-increasing fold-containing family B member 4 (BPIFB4) has been found significantly enriched in long-living individuals. Neuroinflammation is a key player in Huntington's disease (HD), a neurodegenerative disorder caused by neural death due to expanded
    Ho-Ying Wan et al.
    Acta biomaterialia, 140, 163-177 (2021-12-08)
    The development of bone-like tissues in vitro that exhibit key features similar to those in vivo is needed to produce tissue models for drug screening and the study of bone physiology and disease pathogenesis. Extracellular matrix (ECM) is a predominant
    Melanie A Burkhardt et al.
    Biomaterials science, 5(10), 2009-2023 (2017-08-16)
    Blood clots stop bleeding and provide cell-instructive microenvironments. Still, in vitro models used to study implant performance typically neglect any possible interactions of recruited cells with surface-adhering blood clots. Here we study the interaction and synergies of bone marrow derived

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