D0571
Monoclonal Anti-DICER1 antibody produced in mouse
~1.0 mg/mL, clone DCR1, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-DCR1, Anti-HERNA, Anti-Helicase with RNASE motif, Anti-Helicase-MOI, Anti-K12H4.8-LIKE
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DCR1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~218 kDa
species reactivity
human, mouse, rat
concentration
~1.0 mg/mL
technique(s)
western blot: 2.5-5.0 μg/mL using whole extract of human HEK-293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DICER1(23405)
mouse ... Dicer1(192119)
rat ... Dicer1(299284)
General description
Dicer is a member of the RNase III family containing an N-terminal DEXH-box RNA helicase/ATPase domain, followed by a domain of unknown function (DUF283), a PAZ domain, which anchors the 3′-end of the guided siRNA, two RNase III domains, and a dsRBD. The current model for dsRNA cleavage by Dicer predicts that the two ribonuclease III domains of Dicer dimerize to form the catalytic center that is responsible for cleaving long dsRNA in co-operation with two RNA binding domains, PAZ and dsRNA-binding domain.
Monoclonal Anti-DICER1 (mouse IgG1 isotype) is derived from the hybridoma DCR1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human DICER1.
Application
The antibody may be used in several immunological techniques including immunoblotting and real‐time PCR.
Biochem/physiol Actions
Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). The dsRNA is processed into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′ end. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. DICER1-deficient colorectal cancer cells have an enhanced ability to initiate tumors and metastasis. Dicer functions as an indirect tumor suppressor, as silencing Dicer expression makes cancer cells more malignant.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Critical role of the miR-200 family in regulating differentiation and proliferation of neurons
Pandey A, et al.
Journal of Neurochemistry, 133(5), 640-652 (2015)
Impaired DICER1 function promotes stemness and metastasis in colon cancer.
Iliou MS, da Silva-Diz V, Carmona FJ, et al.
Oncogene, 33(30), 4003-4015 (2014)
Yukihide Tomari et al.
Genes & development, 19(5), 517-529 (2005-03-03)
RNA silencing pathways convert the sequence information in long RNA, typically double-stranded RNA, into approximately 21-nt RNA signaling molecules such as small interfering RNAs (siRNAs) and microRNAs (miRNAs). siRNAs and miRNAs provide specificity to protein effector complexes that repress mRNA
Richard I Gregory et al.
Cell, 123(4), 631-640 (2005-11-08)
RNA interference is implemented through the action of the RNA-induced silencing complex (RISC). Although Argonaute2 has been identified as the catalytic center of RISC, the RISC polypeptide composition and assembly using short interfering RNA (siRNA) duplexes has remained elusive. Here
Haruhiko Siomi et al.
Nature, 457(7228), 396-404 (2009-01-23)
The finding that sequence-specific gene silencing occurs in response to the presence of double-stranded RNAs has had an enormous impact on biology, uncovering an unsuspected level of regulation of gene expression. This process, known as RNA interference (RNAi) or RNA
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