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Merck
CN

D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym(s):

3,5-Dinitro-2-hydroxybenzoic acid, DNS

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About This Item

Linear Formula:
(O2N)2C6H2-2-(OH)CO2H
CAS Number:
609-99-4
Molecular Weight:
228.12
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24893243
EC Number:
210-204-3
Beilstein/REAXYS Number:
2220661
MDL number:
MFCD00007104

Key Documents

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Product Name

3,5-Dinitrosalicylic acid, used in colorimetric determination of reducing sugars

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ1057
MKCX9507
MKCX2724
MKCW6704
MKCW4641

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Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Gusakov, A.V., et al.
International Journal of Antimicrobial Agents, e283658-e283658 (2011)
Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.
Mokni-Tlili S, et al.
World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)
Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.
Breuil, C. and Saddler, J.N.
Enzyme and Microbial Technology, 7, 327-332 (1985)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)
Reducing value methods for maltodextrins. I. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper.
J F Robyt et al.
Analytical biochemistry, 45(2), 510-516 (1972-02-01)
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Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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