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D0530

Sigma-Aldrich

Dialysis tubing, high retention seamless cellulose tubing

avg. flat width 32 mm (1.3 in.), MWCO 12400, 99.99% retention

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UNSPSC Code:
41123100
NACRES:
NB.51

avg. diam.

20 mm , when full

avg. flat width

32 mm (1.3 in.)

capacity

~100 mL/ft

pore size

12400 MWCO, 99.99% retention

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General description

Retains >99% of cytochrome c (M.W. 12,400) in solution over a 10-hour period.

Packaging

Supplied in rolls, dry

Quality

May contain glycerin and sulfur compounds in more than trace amounts; washing instructions are included.

Preparation Note

Removal of glycerol included as a humectant can be accomplished by washing the tubing in running water for 3-4 hours. Removal of sulfur compounds can be accomplished by treating the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute. Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid. This tubing will retain most proteins of molecular weight 12,000 or greater.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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