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CYP1

CelLytic™ Y Plus Kit

For enzymatic yeast cell lysis

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About This Item

NACRES:
NA.56
UNSPSC Code:
12352200

Key Documents

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shipped in

dry ice

storage temp.

−20°C

Application

The kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris, and Schizosaccharomyces pombe yeast strains. The procedure employs spheroplast formation by enzymatic digestion of the yeast cell wall followed by cell protein extraction (with or without detergent), while avoiding protein degradation and interference with protein immuno­reactivity and biological activity.
The cell extract is compatible with:
  • Reporter gene expression assays (e.g., β-galactosidase, alkaline-phosphatase)
  • Immunoassays (Western blots, immunoprecipitation)
  • Affinity based purification (e.g. FLAG, glutathione S- transferase (GST) and histidine-tagged fusion proteins)
  • DNA-protein interaction assay (e.g., gel-shift)
  • Coomassie and silver staining
The kit is suitable for spheroplast formation and protein extraction from 10-40 grams of yeast cell pellet.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Reaction Buffer 120 mL
  • 1 M DTT 5 mL
  • Lyticase 25000 U
  • Extraction buffer 60 mL
  • Triton™ X-100 10 mL
  • Protease inhibitor cocktail 2 mL

signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F

flash_point_c

87 °C

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
127M4167V
11171016
047M4115V
11170406
075M4053V

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Articles

Yeast Cell Lysis Reagents

CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.

酵母细胞裂解试剂

我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。

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