Anti-Mouse CD134/OX40 (OX-86) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

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Clone OX-86 is a rat monoclonal antibody that detects CD134/OX40. It targets an epitope within CRD4 from the internal region.

Recombinant mouse OX-40-CD4 chimeric protein.

Quality Control Testing

Evaluated by Flow Cytometry in concanavalin A-stimulated Mouse splenocytes.

Flow Cytometry Analysis: One million BALB/c mouse splenocytes were stimulated with concanavalin A ( 3 µg/mL) for three days. Cells were stained with APC conjugated anti-mouse CD3 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1170, Anti-Mouse CD134/OX40 (OX-86) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1011 Rat IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD134 expression was observed in CD3 positive population.

Tested Applications

Flow Cytometry Analysis: One million BALB/c mouse splenocytes were stimulated with 3 µg/mL of concanavalin A (ConA) for three days. Cells were stained with PE conjugated anti-mouse CD3 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1170, Anti-Mouse CD134/OX40 (OX-86) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or the equivalent amount of Cat. No. CWA-1011 Rat IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD134 expression was observed in CD3 positive population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Tumor necrosis factor receptor superfamily member 4 (UniProt: P47741; also known as OX40 antigen, OX40L receptor, CD134) is encoded by the Tnfrsf4 (also known as Ox40, Txgp1) gene (Gene ID: 22163) in murine species. CD134/OX40 is a TNF-receptor family member that is expressed primarily on activated CD4+ and CD8+ T cells. Its engagement by ligands present on dendritic cells dramatically increases the proliferation, effector function, and survival of T cells. It is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-211), a transmembrane domain (aa 212-236), and a cytoplasmic domain (aa 237-272). It is expressed in CD4+ T-cells and in T-helper Th17 cells. It serves as a receptor for OX40 ligand (OX40L), a co-stimulatory molecule that has been implicated in long-term T-cell immunity. It is also reported to suppress the differentiation and activity of Treg. OX40 and OX40L are also known to regulate cytokine production from T cells, antigen-presenting cells. OX40 contains four cysteine-rich domains (CRD) in its extracellular domain region. Clone OX86 is reported to bind to the CRD4 region; however, it does not block the binding of OX40 ligand. (Ref.: Zhang, P., et al. (2019). Cell Rep. 27(11); 3117-3123; Curti, BD., et al. (2013). Cancer Res. 73(24); 7189-7198; Croft, M., et al. (2009). Immunol. Rev. 229(1); 173-191).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.