Anti-Mouse TIM-4 (RMT4-54) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

T-cell immunoglobulin and mucin domain-containing protein 4 (UniProt: Q6U7R4; also known as TIMD-4, Spleen mucin-containing knockout of lymphotoxin protein, SMUCKLER, T-cell immunoglobulin mucin receptor 4, TIM-4, T-cell membrane protein 4) is encoded by the Timd4 (also known as Tim4) gene (Gene ID: 276891) in murine species. TIM-4 is a single-pass type I, homodimeric membrane protein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-279), a transmembrane domain (aa 280-300), and a cytoplasmic domain (aa 301-343). It also contains an Ig-like type V domain (aa 23-128). It is predominantly expressed in lymphoid tissues, such as spleen, lymph nodes, and in Peyer patches. Its expression is also observed in fetal liver, salivary gland, and spleen stromal cells. TIM-4 serves as a phosphatidylserine (PS) receptor and mediates phagocytosis of apoptotic cells by binding to PS exposed on the surface of these cells. TIM-4 is shown to form a trimeric complex with fibronectin 1 and integrins, which enables efficient recognition of apoptotic cells and mediates their ingestion through the action of integrins. It also acts as a scavenger receptor and mediates phagocytosis of exogenous particles in a phosphatidylserine-independent manner. (Ref.: Min, C., et al. (2020). Cell Death & Disease. 11; Article number: 561; Manzanet, RR., et al. (2008). J. Immunol. 180(7); 4706-4713; Miyanishi, M., et al. (2007). Nature. 450(7168); 435-439).

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Mouse TIM-4-Ig fusion protein

Quality Control Testing

Evaluated by Flow Cytometry in LPS-stimulated mouse macrophages.

Flow Cytometry Analysis: One million BALB/c mouse macrophages were stimulated with 1 μg/mL of LPS for 24 hours and stained with 5 μL of a 1:1 mixture of Cat. No. CWA-1155, Anti-Mouse TIM-4 (RMT4-54) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1018 Rat IgG2a isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: One million BALB/c mouse macrophages were unstimulated (left figure) or stimulated (right figure) with 1 μg/mL of LPS for 24 hours and stained with 5 μL of a 1:1 mixture of Cat. No. CWA-1155, Anti-Mouse TIM-4 (RMT4-54) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1018 Rat IgG2a isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone RMT4-54 is a rat monoclonal antibody that detects Mouse T-cell immunoglobulin and mucin domain-containing protein 4 (TIM-4).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.