Anti-Human Integrin αVβ3 (LM609) ColorWheel^® Dye-Ready mAb

Quality Control Testing

Evaluated by Flow Cytometry in Human umbilical vein endothelial cells (HUVEC).

Flow Cytometry Analysis: Staining of one million Human umbilical vein endothelial cells (HUVEC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1141, Anti-Human Integrin VͰ3 (LM609) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human umbilical vein endothelial cells (HUVEC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1141, Anti-Human Integrin VͰ3 (LM609) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-101 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone LM609 is a recombinant mouse monoclonal antibody that detects human Integrin Vβ3 (CD51/CD61).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

This ColorWheel^® Ready monoclonal antibody was developed using our proprietary ZooMAb^® recombinant antibody technology. It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Integrin Vβ3 (UniProt: P06756/P05106; also known as CD51/CD61) is encoded by the ITGAV/ITGB3 (also known as MSK8, VNRA, VTNR/GP3A) genes (Gene ID: 3685/3690) in human. Integrin V is a single-pass membrane protein that heterodimerizes with a β-subunit. The exact combination of various - and β- subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular region of the -subunit. Integrin V associates with either β1, β3, β5, β6, or β8 in a non-covalent manner. The structural and functional diversity of the integrin family are based upon the pairing abilities of the individual - and β-subunits. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. Integrin Vβ3 serves as a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Both Integrin V and β3 are single-pass membrane proteins that are synthesized with a signal peptide (aa 1-30 and 1-26, respectively), which is subsequently cleaved off to generate the mature form. Their mature forms contain an extracellular domain (aa 31-992/27-718), a transmembrane domain (aa 993-1016/719-741), and a cytoplasmic domain (aa 1017-1048/742-788).

This ColorWheel^® Dye-Ready monoclonal antibody was developed using our ZooMAbZooMAb^® recombinant monoclonal antibody (Cat. No. ZMS1030). It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Full length purified Integrin Vβ3 from human melanoma cell line M21.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany