Anti-Human CD262 (DR5-01-1) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone DR5-01-1 is a mouse monoclonal antibody that detects CD262 (Death Receptor 5). It targets an epitope within the extracellular domain.

Recombinant fusion protein of human IgG heavy chain and extracellular domain of CD269 (Death Receptor 5).

Quality Control Testing

Evaluated by Flow Cytometry in U937 cells.

Flow Cytometry Analysis: Staining of one million U937 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1111, Anti-Human CD262 (DR5-01-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million U937 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1111, Anti-Human CD262 (DR5-01-1) ColorWheel Mouse mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Tumor necrosis factor receptor superfamily member 10B (UniProt: O14763; also known as Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD262) is encoded by the TNFRSF10B (also known as DR5, KILLER, TRAILR2, TRICK2, ZTNFR9) gene (Gene ID: 8795) in human. CD262 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-55), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 56-210), a transmembrane domain (aa 211-231), and a cytoplasmic domain (aa 232-440). It is a pro-apoptotic member of the tumor necrosis factor receptor superfamily that is constitutively expressed in various tissue types and forms auto-inhibited dimers in its resting state. It is widely expressed in adult and fetal tissues and its higher expression is observed in heart, peripheral lymphocytes, liver, pancreas, spleen, placenta, and throughout the intestinal tract. High expression of CD262 is also reported in various cancer cell lines. In its resting state, it is a monomeric protein that serves as a receptor for TRAIL (TNFSF10) and mediates apoptotic cell death. In its canonical mode of activation, binding of its ligand TRAIL assembles it into higher-order oligomers that forms intracellular scaffolds in which its cytosolic death domains recruit the adaptor protein FADD and pro-caspase 8 into the death-inducing signaling complex (DISC). Subsequently, pro-caspase 8 undergoes auto-proteolysis to form active initiator caspase 8. (Ref.: Lam, M., et al. (2020). eLife. 9; e52291; Sun, SY., (2011). Mol. Cell Pharmacol. 3(1); 31-34).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.