Anti-Mouse MHC II (M5/114.15.2) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone M5/114.15.2 is a rat monoclonal antibody that detects MHC Class II; Major Histocompatibility Class II (MHC II).

Activated C57BL/6 mouse spleen cells.

Quality Control Testing

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1081-25UL, Anti-Mouse MHC II (M5/114.15.2) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

H-2 class II histocompatibility antigen, A-K alpha chain/H-2 class II histocompatibility antigen, A beta chain/H-2 class II histocompatibility antigen gamma chain (UniProt: P01910/ P14483/P04441) is encoded by the H2-Aa/H2-Ab1/Cd74 gene (Gene ID: 14960/14961/16149) in murine species. MHC Class II molecules are a class of major histocompatibility complex (MHC) molecules found on antigen-presenting cells such as dendritic cells, mononuclear phagocytes, some endothelial cells, thymic epithelial cells, and B cells. Their major function is to present processed antigens, derived primarily from exogenous sources, to CD4+ T-lymphocytes. MHC-II is a heterodimeric transmembrane glycoprotein comprising an subunit associated non-covalently with a β subunit. In mice two isoforms of MHC Class II have been described and are known as I-A and I-E and they can form A /Aβ and E /Eβ heterodimers. The newly synthesized and β chains assemble into heterodimers in the endoplasmic reticulum, where they are bound by trimers of invariant chain (g), which serves as a dedicated chaperone. They form nonamers of three a/b/g heterotrimers that traffic into late-stage endosomal compartment where the invariant chain is degraded to form CLIP (class II invariant chain-associated peptide). CLIP temporarily occupies the peptide binding groove of the MHC class II molecules. A catalytic protein, HLA-DM, then exchanges CLIP for peptides that bind MHC class II with high-affinity. The peptide/MHC class II complex can then traffic to the cell surface for interaction with CD4+ T cells through the T-cell receptor, which serves as the first signal to the lymphocytes to elicit an antigen-specific adaptive response. Clone M5/114.15.2 reacts with mouse MHC Class II alloantigens I-Ab, I-A d, I-Aq, I-Ed, and I-Ek and can cross-react with mouse cells of H-2p and H-2r haplotype. (Ref.: Wosen, JE., et al. (2018). Front. Immunol. 9; 2144; Holling, TM., et al. (2006). Human Immunol. 65(4); 282-290).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.