CRISPR20V
Lenti CRISPR Universal Non-Target Control#3 Transduction Particles (LV04 vector)
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About This Item
UNSPSC Code:
41106609
NACRES:
NA.51
form
liquid
Quality Level
packaging
vial of 200 μL
concentration
(1x106 TU/mL (via p24 titering assay))
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The Lenti CRISPR Non-Target Control#3 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#3 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Control#3 uses a dual-component system consisting of U6-driven guide RNA (non-target#3) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Articles
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
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