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CLS3650

Corning® 96 well NBS™ Microplate

flat bottom, black polystyrene, bag of 25 ×, non-sterile, lid: no

Synonym(s):

96 multiwell plates, 96 well microplates, 96 well microtiter plates, 96 well plates

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About This Item

UNSPSC Code:
41121800
NACRES:
NB.13

material

black polystyrene
flat bottom
polystyrene
round clear wells

sterility

non-sterile
non-sterile

feature

lid: no
lid: no
skirt
 plate format: 96 well standard

packaging

case of 100
pack of 25
bag of 25 ×

manufacturer/tradename

Corning 3650

maximum volume

360 μL

size

96 wells

working volume

200 μL

color

black

binding type

NBS™ (non-binding surface)

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General description

  • Bottoms are 60% thinner than conventional polystyrene plates, resulting in lower background fluorescent and enabling readings down to 340nm
  • Designed to reduce well-to-well crosstalk during fluorescent assays
  • Nonbinding (NBS) surface is a Corning proprietary treatment technology used on polystyrene microplates to create a nonionic hydrophilic surface (polyethylene oxide-like) that minimizes molecular interactions
  • Ideal for reducing protein and nucleic acid binding at low concentrations, and increasing assay signal to noise
  • Without lids, lids supplied separately
  • Recommended working volume of 75 to 200μL
  • Conforms to standard microplate footprint and dimensions
Non-Binding surface (NBS) multiwell plates have a modified polymer surface (PS) which results in a non-ionic hydrophilic surface (polyethylene oxide-like), thereby minimizing bio-interaction and enhancing the signal-to-noise ratio. Results have demonstrated NBS′s ability to significantly reduce protein and nucleic acid binding to polymers (<2 ng/cm2; over 90% reduction of protein binding compare to PS & PP surface), maintain enzyme activity, and inhibit adhesion of a number of cell lines. These novel surface technologies for assay plates are ideal for homogeneous assays such as Scintillation Proximity Assay (SPA). In addition to a 20+ fold increase in signal-to-noise ratio, selected assays have also displayed improved sensitivity and specificity. The recommended working volume of up to 75 μL to 200 μL for 96 well plates and 20μL to 80 μL for 384 well plates.

Legal Information

Corning is a registered trademark of Corning, Inc.
NBS is a trademark of Corning, Inc.

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Ujwal Patil et al.
Biosensors & bioelectronics, 165, 112327-112327 (2020-07-31)
Purification of therapeutic monoclonal antibodies usually involves a protein A affinity capture step. Because column breakthrough of antibody in complex, UV-absorbing culture fluid cannot be readily detected in real time, processes are designed so conservatively that column capacity is usually

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