CLLS1004
DLD1 Cells PTEN -/-
human male colon (Source Disease: Colorectal adenocarcinoma)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
product name
DLD1 Cells PTEN -/-,
biological source
human male colon (Source Disease: Colorectal adenocarcinoma)
Quality Level
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... PTEN(5728)
General description
DLD1 Cells PTEN -/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.
This product corresponds to ATCC Cat. No.CCL-221
Application
Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:10
The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Features and Benefits
These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 10q23.31
Quality
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Regulatory Information
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Certificates of Analysis (COA)
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Xi Kang et al.
Scientific reports, 5, 17873-17873 (2015-12-15)
PTEN is a powerful tumor suppressor that antagonizes the cytoplasmic PI3K-AKT pathway and suppresses cellular proliferation. PTEN also plays a role in the maintenance of genomic stability in the nucleus. Here we report that PTEN facilitates DNA decatenation and controls
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