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CHOZN® ZFN-Modified CHO Cell Line

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100 ML
CN¥1,272.36
4 X 100 G
CN¥1,955.08
500 ML
CN¥3,041.11
1 L
CN¥7,426.53

CN¥1,272.36

Estimated to ship onJune 12, 2025Details

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100 ML
CN¥1,272.36
4 X 100 G
CN¥1,955.08
500 ML
CN¥3,041.11
1 L
CN¥7,426.53

About This Item

UNSPSC Code:
12352200
NACRES:
NA.75

CN¥1,272.36

Estimated to ship onJune 12, 2025Details

description

CHOZN® GS-/- ZFN-modified CHO cell line
glutamine synthetase knockout cell line

solubility

DMSO: 5 mg/mL, clear

shipped in

liquid nitrogen

storage temp.

−196°C

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1 of 4

This Item
CLS431144CLS430421CLS430183
Corning® Erlenmeyer cell culture flasks 125 mL Erlenmeyer Flask w/ Vent Cap, polycarbonate, sterile

CLS431143

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 250 mL Erlenmeyer Flask w/ Vent Cap, polycarbonate, sterile

CLS431144

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 125 mL Erlenmeyer Flask w/ Flat Cap, polycarbonate, sterile, 50/cs

CLS430421

Corning® Erlenmeyer cell culture flasks

Corning® Erlenmeyer cell culture flasks 250 mL Erlenmeyer Flask w/ Flat Cap, polycarbonate, sterile, 50/cs

CLS430183

Corning® Erlenmeyer cell culture flasks

manufacturer/tradename

Corning 431143

manufacturer/tradename

Corning 431144

manufacturer/tradename

Corning 430421

manufacturer/tradename

Corning 430183

material

clear polycarbonate flask, polypropylene cap (Easy-Grip vent), wide-mouth , flat bottom flask, wide-mouth neck, vent cap

material

clear polycarbonate flask, flat bottom flask, polypropylene cap (Easy-Grip vent), vent cap, wide-mouth , wide-mouth neck

material

clear polycarbonate flask, flat bottom flask, flat cap, polypropylene cap (Easy-Grip flat), wide-mouth , wide-mouth neck

material

clear polycarbonate flask, flat bottom flask, flat cap, polypropylene cap (Easy-Grip flat), wide-mouth , wide-mouth neck

feature

cap, graduations 25 mL, disposable, no baffles

feature

disposable, graduations 25 mL, with cap, no baffles

feature

cap, disposable, graduations 25 mL, no baffles

feature

cap, disposable, graduations 25 mL, no baffles

packaging

pack of 1

packaging

pack of 1, case of 50

packaging

pack of 1, case of 50

packaging

pack of 1, case of 50

flask capacity

125 mL

flask capacity

250 mL

flask capacity

125 mL

flask capacity

250 mL

General description

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media
ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus.[1] Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth.[2] The GS enzyme is responsible for the conversion of glutamate into glutamine.[3] Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Cell Line Origin

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

Packaging

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Physical form

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Legal Information

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源生物产品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    2113-53667
    051M0924

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    Find documentation for the products that you have recently purchased in the Document Library.

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    The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type.
    Yuneva M O, et al.
    Cell Metabolism, 15(2), 157-170 (2012)
    Cytotoxic effects during knock out of multiple porcine endogenous retrovirus (PERV) sequences in the pig genome by zinc finger nucleases (ZFN).
    Semaan M, et al.
    PLoS ONE, 10(4), e0122059-e0122059 (2015)
    Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer.
    DeBerardinis R J and Cheng T
    Oncogene, 29(3), 313-313 (2010)

    Articles

    Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing

    Understand how considerations such as DMSO concentrations and freezing techniques are key in the successful implementation of high cell density cryopreservation (HCDC).

    Optimizing CHO Cells for Higher Productivity

    Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

    Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

    In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

    Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

    MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

    See All

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