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CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Synonym(s):

Mitochondrial activity assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

packaging

pkg of 1 kit

Quality Level

200

technique(s)

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

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Product No.
Description
Pricing

Signal Word

Danger

Hazard Statements

H225,H319,H336,H341

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can we use media with phenol red with Product CGD1, Cell Growth Determination Kit?

    You most likely would not be able to use a medium containing phenol red with this kit. We have found that the phenol red can contribute to higher background, and therefore lower sensitivity.

  4. What is the difference between Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?

    Product No. CGD1 and TOX1 are both MTT based assays. The major difference between Product No. CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT.  If there is complete cell lysis, the results obtained with both kits would be the same.  The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.

  5. What are the components of the Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?

    CGD1 contains 5 vials of 5 mg/vial of MTT and 50 ml of MTT solvent (solubilization solution) and TOX1 contains 5 vials of 15 mg/vial of MTT and 125 ml of solubilization solution.

  6. Is 10% fetal bovine serum (FBS) in the culture medium compatible with Product CGD1, Cell Growth Determination Kit?

    High protein levels may form a precipitate when the MTT solubilization solution is added. Samples with protein concentrations equivalent to 10% FBS or 4 mg/mL protein seem acceptable.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Is the MTT assay quantitative?

    The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.   

  10. What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?

    Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT.  If there is complete cell lysis, the results obtained with both kits would be the same.  The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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