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Safety Information

CG201

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit

sufficient for 96 assays

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100 STRIPS
¥207.41

¥207.41

Estimated to ship on2025年4月15日Details

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100 STRIPS
¥207.41

About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

¥207.41

Estimated to ship on2025年4月15日Details

usage

sufficient for 96 assays

Quality Level

200

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592)

General description

cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The immunoassay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

Application

Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues[1] and mouse aortic artery homogenates.[2] It has also been used to assess the guanylate cyclase activity.[3]

Kit Components Only

Product No.
Description
  • Acetic Anhydride 1 x 1
  • Assay Buffer 2 1 x 30
  • cGMP-Alkaline Phosphatase Conjugate 1 x 5
  • cGMP Assay Layout Sheet 1 x 1
  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
  • 5 Cycle Logit-Log Paper 1 x 1
  • Cyclic GMP Standard 1 x 0.5
  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20
  • Plate Sealer 1 ea
  • Stop Solution 1 x 5
  • Triethylamine 1 x 2
  • Wash Buffer Concentrate 1 x 30
See All (13)

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

12.2 °F - closed cup

Flash Point(C)

-11 °C - closed cup

Regulatory Information

危险化学品
常规特殊物品
易制毒化学品（2类）
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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCL9470
    SLCN6462
    SLCK4715
    SLCJ8784
    SLCG5381

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    Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro
    Mulaudzi T, et al.
    Febs Letters, 585(17), 2693-2697 (2011)
    Inhibition of MPO (Myeloperoxidase) Attenuates Endothelial Dysfunction in Mouse Models of Vascular Inflammation and Atherosclerosis
    Cheng D, et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology, 39(7), 1448-1457 (2019)
    David Cheng et al.
    Arteriosclerosis, thrombosis, and vascular biology, 39(7), 1448-1457 (2019-05-03)
    Objective- Inflammation-driven endothelial dysfunction initiates and contributes to the progression of atherosclerosis, and MPO (myeloperoxidase) has been implicated as a potential culprit. On release by circulating phagocytes, MPO is thought to contribute to endothelial dysfunction by limiting NO bioavailability via
    Genistein from Flemingia vestita (Fabaceae) enhances NO and its mediator (cGMP) production in a cestode parasite, Raillietina echinobothrida
    Das B, et al.
    Parasitoloty, 134(10), 1457-1463 (2007)
    Kamal Kumar Malukani et al.
    Plant physiology, 183(3), 1345-1363 (2020-05-02)
    Plant pathogens secrete cell wall-degrading enzymes that degrade various components of the plant cell wall. Plants sense this cell wall damage as a mark of infection and induce immune responses. However, the plant functions that are involved in the elaboration
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