CE0050
CelLytic™ MEM Protein Extraction Kit
Synonym(s):
Membrane protein extraction kit
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About This Item
NACRES:
NA.56
UNSPSC Code:
41116134
General description
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
Application
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Sufficient reagents supplied for 80 tests.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
signalword
Danger
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
Regulatory Information
常规特殊物品
低风险生物材料
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Willy Morelle et al.
The Journal of clinical endocrinology and metabolism, 102(4), 1375-1386 (2017-03-23)
TMEM165 deficiency is a severe multisystem disease that manifests with metabolic, endocrine, and skeletal involvement. It leads to one type of congenital disorders of glycosylation (CDG), a rapidly growing group of inherited diseases in which the glycosylation process is altered.
Tomoaki Furuta et al.
Cancer science, 112(9), 3722-3731 (2021-06-12)
The rBC2LCN lectin, known as a stem cell marker probe that binds to an H type 3 fucosylated trisaccharide motif, was recently revealed to also bind to pancreatic ductal adenocarcinoma (PDAC) cells. A lectin-drug conjugate was generated by fusing rBC2LCN
Hiroaki Tateno et al.
Glycobiology, 26(12), 1328-1337 (2016-04-03)
Human somatic stem cells such as human mesenchymal stem cells (hMSCs) are considered attractive cell sources for stem cell-based therapy. However, quality control issues have been raised concerning their safety and efficacy. Here we used lectin microarray technology to identify
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CE0050-1KT | 04061835518050 |