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LNCap Clone FGC Cell Line human

89110211, human prostate (carcinoma), Epithelial-like

Synonym(s):

LNCaP-FGC Cells

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About This Item

UNSPSC Code:
41106514

product name

LNCap Clone FGC Cell Line human, from human prostate(carcinoma), 89110211

biological source

human prostate (carcinoma)

growth mode

Adherent

karyotype

Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91

morphology

Epithelial-like

products

Prostate acid phosphatase, prostate specific antigen

receptors

Androgen, estrogen

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian prostate carcinoma

Cell Line Description

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Application

LNCap Clone FGC Cell Line has been used:
  • to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
  • to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
  • to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

Culture Medium

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm2 Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  6. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  7. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

  8. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

  9. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  10. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

  11. How to freeze down Cell Line cells to make a cell stock?

    A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

  12. Are the Cell Line cells tested for viral pathogens?

    HPA Cultures does not perform any viral testing on the cell lines.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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