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Key Documents

Safety Information

THP 1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88081201, human blood, Monocyte

Synonym(s):

O-THP-1 Cells, THP-1 Macrophages, THP-1 Monocytes, THP1 Cells, THPI Cells, Tohoku Hospital Pediatrics-1 Cells

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About This Item

UNSPSC Code:
41106514

biological source

human blood

packaging

tube of 5 μg 88081201-DNA-5UG
pkg of vial of cells 88081201-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Monocyte

products

Not specified

receptors

Fc, C3b

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

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Show Differences

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PSF-RSV - RSV PROMOTER PLASMID plasmid vector for molecular cloning

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PSF-SV40 - SV40 PROMOTER PLASMID plasmid vector for molecular cloning

OGS47

PSF-SV40 - SV40 PROMOTER PLASMID

PSF-PGK - PHOSPHOGLYCERATE KINASE PROMOTER PLASMID plasmid vector for molecular cloning

OGS403

PSF-PGK - PHOSPHOGLYCERATE KINASE PROMOTER PLASMID

PSF-CMV-RSV-BLAST ASCI - RSV PROMOTER BLASTICIDIN PLASMID plasmid vector for molecular cloning

OGS383

PSF-CMV-RSV-BLAST ASCI - RSV PROMOTER BLASTICIDIN PLASMID

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

peptide cleavage

no cleavage

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

origin of replication

pUC (500 copies)

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

bacteria selection

kanamycin

mol wt

size 4122 bp

mol wt

size 3867 bp

mol wt

size 4172 bp

mol wt

size 5224 bp

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

Cell Line Origin

Human monocytic leukaemia

Cell Line Description

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for α-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

Application

THP 1 cell line human has been used to study:
  • bactericidal activity of moxifloxacin against Staphylococcus aureus[1]
  • the effect of lipopolysaccharide-mediated inflammatory response in murine versus human cell lines[2]
  • the effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum[3]
  • the change in gene expression after infection with Mycobacterium tuberculosis[4]
  • the induction of cytokines in the cell line in presence of antigens obtained from Clostridium difficile[5]

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS / FCS).

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% fetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. How do I handle Cell Line frozen cells upon arrival?

      Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

    5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

      If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

    6. How do I resuscitate/thaw frozen Cell Line cells?

      A protocol for thawing cells can be found in the ECACC handbook.

    7. What medium should I use when I thaw the Cell Line cells?

      The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

    8. What passage are my Cell Line cells?

      If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

    9. How many Cell Line cells are in the vial?

      Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

    10. Are my Cell Line cells mycoplasma tested?

      ECACC routinely tests all manufactured cell banks for mycoplasma.

    11. How to freeze down Cell Line cells to make a cell stock?

      A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

    12. Are the Cell Line cells tested for viral pathogens?

      HPA Cultures does not perform any viral testing on the cell lines.

    13. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Articles

    ECACC Nucleic Acids

    DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service