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Safety Information

WEHI 164 Cell Line from mouse

87022501, mouse skin (fibroblast), Fibroblast

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About This Item

UNSPSC Code:
41106514

Product Name

WEHI 164 Cell Line from mouse, from mouse skin(fibroblast), 87022501

biological source

mouse skin (fibroblast)

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse BALB/c fibrosarcoma

Cell Line Description

Established from a fibrosarcoma induced by subcutaneous injection of methylcholanthrene in BALB/c mice. Highly sensitive after pre-treatment with actinomycin D to human cytotoxic monocytes, to tumour necrosis factor and to lymphotoxin. It provides convenient target cells for short-term (51) chromium release assays.

Application

Tumourigenicity and cytotoxicity studies, chromium release assays

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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