C9995
Anti-Cullin-4B antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-Cul4B, Anti-MRXHF2, Anti-MRXSC, Anti-SFM2
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CUL4B(8450)
mouse ... Cul4b(72584)
rat ... Cul4b(302502)
Related Categories
General description
Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B. Cul4A and Cul4B form individual E3 ligases. In contrast to Cul4A, Cul4B has been shown to be localized to the nucleus.
Application
Anti-Cullin-4B antibody produced in rabbit is suitable for western blotting at a working concentration of 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. The antibody was used for immunoblotting in a study to demonstrate that CUL4B E3 ubiquitin ligase plays a key role in targeting Jab1 (Jun activation domain-binding protein) for degradation and regulates bone morphogenetic protein signaling.
Biochem/physiol Actions
Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases that regulate cell cycle, genome stability and DNA replication. Cul4B functions as an E3 ligase involved in selecting specific targets for ubiquitylation. It associates with damage-specific DNA binding proteins DDB1 and DDB2 (UV-damaged DNA) and functions as a substrate-recruiting factor for Cul4B E3 ligases. Cul4B ubiquitylates several target proteins including β-catenin, cyclin E, and histone H2A Mutations in this gene has been associated with x-linked mental retardation.
Cullin-4B facilitates the transfer of damaged-DNA binding protein 1 (DDB1) into the nucleus independently of DDB2. In contrast to cullin-4A (Cul4A), cullin-4B (Cul4B) does not ubiquitinate DDB2.
Target description
Cullin-4B forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells
Nag A, et al.
Molecular and Cellular Biology, 21(20), 6738-6747 (2001)
Pengyu Li et al.
Oncology letters, 14(2), 1265-1274 (2017-08-16)
Cullin 4B (Cul4B), a scaffold protein that assembles the ubiquitin ligase complex, is involved in a wide variety of physiological and developmental processes, such as cell cycle progression, DNA damage response and gene expression regulation. Cul4B is overexpressed in various
Hong Lin et al.
Nature communications, 12(1), 2461-2461 (2021-04-30)
COP1 and COP9 signalosome (CSN) are the substrate receptor and deneddylase of CRL4 E3 ligase, respectively. How they functionally interact remains unclear. Here, we uncover COP1-CSN antagonism during glucose-induced insulin secretion. Heterozygous Csn2WT/K70E mice with partially disrupted binding of IP6
Yanjun Li et al.
Oncogenesis, 9(2), 20-20 (2020-02-15)
Given that colorectal cancer stem cells (CCSCs) play key roles in the tumor dormancy, metastasis, and relapse, targeting CCSCs is a promising strategy in cancer therapy. Here, we aimed to identify the new regulators of CCSCs and found that Cullin
Rachana Tripathi et al.
Journal of biosciences, 30(3), 329-337 (2005-07-30)
Heat induced differentiation of mouse embryonal carcinoma cells PCC4 has been reported earlier. We have further characterized the phenotype of the differentiated cells and by DD-RT-PCR identified several partial cDNAs that are differentially expressed during differentiation. Nucleotide homology search revealed
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