Skip to Content
Merck
CN
All Photos(2)

Documents

C9426

Sigma-Aldrich

CHAPS hydrate

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
331717-45-4
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
MFCD00012116
UNSPSC Code:
12161902
PubChem Substance ID:
24892997
NACRES:
NB.22

description

zwitterionic

Quality Level

200

product line

BioReagent

Assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

electrophoresis: suitable
protein quantification: suitable

impurities

<0.04% DMF

mp

157 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear, colorless

suitability

suitable for electrophoresis

storage temp.

room temp

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:
  • For membrane permeabilization of cells
  • As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
  • As a component in denaturing buffer in enzyme characterization
  • As a component of 2D- sample buffer in 2D electrophoresis
  • For protein solubilization in isoelectric focusing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H360D

Hazard Classifications

Acute Tox. 4 Oral - Repr. 1B

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Non-autophagic GFP-LC3 puncta induced by saponin and other detergents
Ciechomska, et al.
Autophagy, 3(6), 586-590 (2007)
Effect of copper nanoparticles exposure in the physiology of the common carp (Cyprinus carpio): Biochemical, histological and proteomic approaches.
Gupta Y, et al.
Aquaculture and Fisheries, 1, 15-23 (2016)
Phytase activity in sourdough lactic acid bacteria: purification and characterization of a phytase from Lactobacillus sanfranciscensis CB1.
De Angelis M, et al.
International Journal of Food Microbiology, 87(3), 259-270 (2003)
Investigation of protein-tyrosine phosphatases by in-gel assays.
Markova B, et al.
Methods, 35(1), 22-27 (2005)
Qingchuan Zhao et al.
Cancer immunology research, 8(4), 544-555 (2020-02-13)
High-grade serous ovarian cancer (HGSC), the principal cause of death from gynecologic malignancies in the world, has not significantly benefited from advances in cancer immunotherapy. Although HGSC infiltration by lymphocytes correlates with superior survival, the nature of antigens that can
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service