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C9301

Sigma-Aldrich

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

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CAS Number:
9007-34-5
EC Number:
232-697-4
MDL number:
MFCD00130825
UNSPSC Code:
12352202
NACRES:
NA.75

product name

Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture

biological source

chicken (Sternal cartilage)

Quality Level

300

type

Type II (Miller)

product line

BioReagent

form

powder

packaging

glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg

concentration

60-80% (biuret)

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

UniProt accession no.

P02460

Binding Specificity

Peptide Source: Fibrinogen

shipped in

wet ice

storage temp.

2-8°C

Gene Information

chicken ... COL2A1(395069)

Related Categories

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used:
  • in enzyme–linked immunosorbent assay (ELISA).
  • chondrocyte-mediated tissue production in vitro.
  • induction, treatment and assessment of collagenα induced arthritis (CIA).
  • cell proliferation assay.
  • as a coating for cell culture surfaces.

Biochem/physiol Actions

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Features and Benefits

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering
Toh W S
Methods in Molecular Biology (2014)
Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.
Hoek R M, et al.
Arthritis and Rheumatism, 62(4), 1036-1042 (2010)
High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes.
Chen X, et al.
Cardiovascular Diabetology, 16(1), 121-121 (2017)
Blockade of the interleukin?7 receptor inhibits collagen?induced arthritis and is associated with reduction of T cell activity and proinflammatory mediators.
Hartgring S A, et al.
Arthritis and Rheumatism, 62(9), 2716-2725 (2010)
Lipopolysaccharide Increases the Incidence of Collagen?Induced Arthritis in Mice Through Induction of Protease HTRA?1 Expression.
Hou Y, et al.
Arthritis and Rheumatism, 65(11), 2835-2846 (2013)
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