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C9142

Sigma-Aldrich

Cyanogen bromide-activated-Sepharose™ 4B

lyophilized powder

Synonym(s):

Cyanogen bromide-activated Agarose

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About This Item

CAS Number:
68987-32-6
MDL number:
MFCD00163385
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 4-5 mL

particle size

45—165 μm

storage temp.

2-8°C

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General description

Cyanogen bromide-activated-Sepharose™ is suitable for use in affinity chromatography and protein chromatography. It has been used to discover insecticidal agents, raise polyclonal antibodies against yeast invertase glycosyls, and study immunological processes of xenograft rejection.

C9142-15G′s updated product number is GE17-0430-01

Application

Cyanogen bromide-activated-Sepharose™ 4B has been used:
  • in the purification of clusterin from serum by an immunoaffinity-based separation procedure
  • in affinity chromatography to isolate Leishmania donovani protein antigens from the urine of visceral leishmaniasis patients
  • in affinity chromatography for the isolation of haptoglobin from human serum

Physical form

Lyophilized powder stabilized with lactose and dextran

Legal Information

Sepharose is a trademark of Cytiva

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Description
Pricing

replaced by

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How are proteins attached to the Cyanogen bromide-activated Sepharose® in Product C9142?

    Coupling of molecules to CNBr-activated Sepharose® is usually via amine groups.  In the case of proteins, this usually means the epsilon amines (exocyclic amines) of lysine groups.

  4. After I've washed and prepared the resin in Product C9142, Cyanogen bromide-activated-Sepharose®, for the coupling reaction, how long can I wait before coupling the ligand to the resin?

    We would recommend coupling the ligand immediately after washing and preparing the resin.  This is because the reactive groups hydrolyze in the basic solution conditions of the preparation steps.  The solutions used in the coupling reaction should also be kept cold (2-8°C) during the procedure.

  5. What kind of volume should I use for my ligand to couple to the Cyanogen bromide-activated Sepharose®, Product C9142?

    It is best that the coupling ligand should not be in too dilute a solution.  In general, a volume ratio of 0.5 to 1 of coupling solution to CNBr-activated Sepharose® is recommended.

  6. My ligand is in Tris buffer.  Can I use Product C9142, Cyanogen bromide-activated-Sepharose®, with this sample?

    Because Tris is an amine-containing buffer, we do not recommend coupling of samples in Tris buffer to the CNBr-activated Sepharose®.  Tris will react with the CNBr groups and remove sites for ligand coupling.  We would not recommend use of any amine-containing buffer in the coupling reaction.  However, after the ligand has been added in the coupling reaction, then it is OK to use amine-containing buffers to block the remaining sites (e.g. ethanolamine, glycine).

  7. When using Product C9142, Cyanogen bromide-activated-Sepharose®, is there a particular storage buffer that I should use after I've coupled my ligand to the resin?

    This depends on the particular situation, and the answer depends more on the coupled ligand than the resin itself.  The most important criterion is to choose a buffer that is not harmful to the ligand.  It is common to include 20% ethanol or preservatives like sodium azide or thimerosal (~0.02%) to inhibit potential bacterial growth.

  8. Should I use a desiccator environment for storage of Cyanogen bromide-activated-Sepharose®?

    It is a good idea to store the CNBr-activated agarose products in as dry an environment as possible, because the reactive groups are very moisture-sensitive.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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