C9080
Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
clone V9, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Vimentin
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V9, monoclonal
form
buffered aqueous solution
mol wt
antigen ~58 kDa
species reactivity
pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
technique(s)
direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VIM(7431)
rat ... Vim(81818)
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.
Specificity
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
Immunogen
pig eye lens vimentin.
Application
Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
- immunofluorescence and confocal microscopy
- cytoskeleton staining
- double labeling experiments
- immunohistochemical
- immunocytochemical localozation
Biochem/physiol Actions
Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Storage and Stability
Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Guangheng Li et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 20(9), 1611-1623 (2005-08-02)
After intramuscular implantation, BMP4-expressing NIH/3T3 fibroblasts and BMP4-expressing C2C12 myoblasts can promote ectopic cartilage and bone formation. Fibroblasts tend to undergo chondrogenesis, whereas myoblasts primarily undergo osteogenesis. These results suggest that endochondral bone formation may involve different cell types, a
Coexpression of keratin-and vimentin-type intermediate filaments in human metastatic carcinoma cells
Ramaekers FC, et al.
Proceedings of the National Academy of Sciences of the USA, 80(9), 2618-2622 (1983)
Yimu Zhao et al.
Matrix biology : journal of the International Society for Matrix Biology, 85-86, 189-204 (2019-04-15)
Organ-on-a-chip systems have the potential to revolutionize drug screening and disease modeling through the use of human stem cell-derived cardiomyocytes. The predictive power of these tissue models critically depends on the functional assembly and maturation of human cells that are
Sven Otto et al.
PloS one, 16(5), e0251530-e0251530 (2021-05-21)
Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads
Morphology of the myoepithelial cell: immunohistochemical characterization from resting to motile phase
Beha G, et al.
TheScientificWorldJournal, 2012 (2012)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service