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C8831

Sigma-Aldrich

Anti-Cyclin B1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-CCNB
MDL number:
MFCD00243104
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 62 kDa

species reactivity

human, mouse, hamster

technique(s)

indirect immunofluorescence: 1:30
microarray: suitable
western blot: 1:100

UniProt accession no.

P14635

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CCNB1(891)
mouse ... Ccnb1(268697)

General description

The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells.

Immunogen

recombinant hamster cyclin B1

Application

Anti-cyclin B1 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex play a crucial role in regulation of mitosis. Elevated expression of the gene has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines. In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER+) breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Importance of tuberin in carcinogenesis.
Jin M, et al.
Oncology Letters, 14, 2598-2602 (2017)
Virus infection disturbs cyclin expression, leading to cell cycle arrest in the unicellular marine algae Emiliania huxleyi and Chrysochromulina ericina
Liu J, et al.
African Journal of Microbiology Research, 5, 1801-1807 (2011)
CCNB1 is a prognostic biomarker for ER+ breast cancer
Ding K, et al.
Medical Hypotheses, 83, 359-364 (2014)
Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer
Fang Y, et al.
Cancer Biology & Therapy, 15, 1268-1279 (2014)
Marie Morikawa et al.
The Journal of reproduction and development, 53(6), 1151-1157 (2007-08-19)
A high cyclic adenosine monophosphate (cAMP) level in fully-grown immature oocytes prevents meiotic resumption. In Xenopus, inhibitory cAMP is synthesized within oocytes depending on a stimulatory alpha-subunit of G-protein (Gsalpha). In the present study, we examined whether ooplasmic Gsalpha is
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