C8526
Chymopapain from papaya latex
lyophilized powder, ≥2.0 units/mg protein
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form
lyophilized powder
specific activity
≥2.0 units/mg protein
mol wt
27 kDa
composition
Protein, ≥75% E1%/280
solubility
H2O: soluble 5.0 mg/mL, clear, colorless
storage temp.
−20°C
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Application
Chymopapain from papaya latex has been used in a study to assess the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides. Chymopapain from papaya latex has also been used in a study to investigate the preparation and identification of the rabbit′s antibody of chymopapain.
The enzyme has been used to detach cultured CD34+ cells from beads (130 units/mL for 2 minutes, repeated three times). Chymopapain and pronase have been found to be effective in the digestion of human intestinal tissue. The enzyme has been used along with pronase, collagenase, elastase, DNAse, and catalase for rabbit lung cells digestion at a concentration of 0.05% in calcium-magnesium-free Kreb′s serum substitute. It has also been used for the release of neuroblastoma cells from marrow bound to antibody-coated microspheres.
Biochem/physiol Actions
The enzyme cleaves amide linkages at the carboxyl side of arginine, leucine, glutamic acid, and alanine residues; as well as that of arginine esters. The optimum pH varies depending on the kind of substrate. For example, the pH optimum for casein digestion is 7.9, hemoglobin digestion is 4.0 and urea-denatured hemoglobin digestion is 7.0. Molecular mass of the enzyme is found to be 27 KDa.
Plant cysteine proteinase that is similar in function to lysosomal cathepsin L.
Other Notes
Contains L-cysteine and EDTA
Unit Definition
One unit will hydrolyze 1.0 μmole of BAEE to Nα-benzoyl-L-arginine per min at pH 6.2 at 25 °C
Preparation Note
Chromatographically purified
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Dispersal of rabbit lung into individual viable cells: a new model for the study of lung metabolism.
K G Gould et al.
Science (New York, N.Y.), 178(4066), 1209-1210 (1972-12-15)
This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed
Y Gazitt et al.
Cancer research, 52(10), 2957-2965 (1992-05-15)
A methodology for rapid isolation of neuroblastoma cells from marrow with metastatic neuroblastoma cells was developed using a cocktail of five antibodies and magnetic microspheres coated with secondary antibodies. Cells bound to microspheres were released by brief exposure to chymopapain
Chymopapain. I. Isolation, crystallization, and preliminary characterization.
M EBATA et al.
The Journal of biological chemistry, 237, 1086-1094 (1962-04-01)
Silas P Rodrigues et al.
Journal of proteomics, 75(11), 3191-3198 (2012-04-03)
Papaya meleira virus (PMeV) is so far the only described laticifer-infecting virus, the causal agent of papaya (Carica papaya L.) sticky disease. The effects of PMeV on the laticifers' regulatory network were addressed here through the proteomic analysis of papaya
Sufang Wang et al.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 25(12), 891-893 (2003-04-11)
Chymopapain, one of the four cysteine proteinases of papaya latex, has milk clotting and proteolytic activity. It is mainly used to treat prolapsed intervertebral discs. This paper introduced the preparation of polyclonal antibody of partially purified chymopapain and the identification
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