C8487

Anti-Caspase 3, Active antibody produced in rabbit

Name: Anti-Caspase 3, Active antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama

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About This Item

MDL number:

MFCD01321906

UNSPSC Code:

12352203

NACRES:

NA.41

Key Documents

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, bovine, human, pig, canine, mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine
microarray: suitable
western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)

General description

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.

Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.

Immunogen

Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.

Application

Anti-Caspase 3, Active antibody has been used:

for western blotting of cytochrome c for caspase activation
as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
in Western blot analysis of activated caspase 3
as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions

Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000508965

0000475628

0000445867

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Caspases: an apoptosis mediator

Palai T K and Mishra S R

Journal of Advanced Veterinary and Animal Research, 2(1) (2015)

Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma

Liu PF, et al.

Testing, 12(7) (2017)

Acidic extracellular pH shifts colorectal cancer cell death from apoptosis to necrosis upon exposure to propionate and acetate, major end-products of the human probiotic propionibacteria.

Annaïg Lan et al.

Apoptosis : an international journal on programmed cell death, 12(3), 573-591 (2006-12-30)

The human probiotic Propionibacterium freudenreichii kills colorectal adenocarcinoma cells through apoptosis in vitro via its metabolites, the short chain fatty acids (SCFA), acetate and propionate. However, the precise mechanisms, the kinetics of cellular events and the impact of environmental factors

Ezh2-mediated repression of a transcriptional pathway upstream of Mmp9 maintains integrity of the developing vasculature.

Paul Delgado-Olguín et al.

Development (Cambridge, England), 141(23), 4610-4617 (2014-11-02)

Maintenance of vascular integrity is required for embryogenesis and organ homeostasis. However, the gene expression programs that stabilize blood vessels are poorly understood. Here, we show that the histone methyltransferase Ezh2 maintains integrity of the developing vasculature by repressing a

Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice.

K Kuida et al.

Nature, 384(6607), 368-372 (1996-11-28)

Programmed cell death (apoptosis) is a prominent feature of the development of the immune and nervous systems. The identification of the Caenorhabditis elegans cell death gene, ced-3, as a prototype of the interleukin-1beta converting enzyme (ICE) protease family has led

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Anti-Caspase 3, Active antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Select a Size

About This Item

Key Documents

Properties

biological source

Quality Level

conjugate

antibody form

antibody product type

clone

form

species reactivity

packaging

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Gene Information

Description

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Disclaimer

Safety Information

Storage Class Code

WGK

Flash Point(F)

Flash Point(C)

Regulatory Information

Documentation

Certificates of Analysis (COA)

Already Own This Product?

Peer Reviewed Papers

Protocols and Articles

Related Content

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