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C8093

Sigma-Aldrich

Monoclonal Anti-Connexin-43 antibody produced in mouse

clone CXN-6, ascites fluid

Synonym(s):

Mouse monoclonal antibody

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About This Item

MDL number:
MFCD00803532
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CXN-6, monoclonal

mol wt

antigen 43 kDa

contains

15 mM sodium azide

species reactivity

rat, hamster, bovine, mouse, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:8,000-1:16.000 using mouse whole brain extract

isotype

IgM

UniProt accession no.

P17302

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GJA1(2697) , GJA1P1(2698)
mouse ... Gja1(14609)
rat ... Gja1(24392)

General description

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.
The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.

Specificity

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

Immunogen

synthetic connexin-43 peptide (362-381).

Application

Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:
  • fluorescent immunostaining
  • western blotting
  • immunocytochemistry
  • preparation of immuno-affinity columns

Biochem/physiol Actions

Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Faten Shaeib et al.
Reproductive sciences (Thousand Oaks, Calif.), 23(4), 498-507 (2015-10-16)
We investigated the ability of reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)), hydroxyl radical ((·)OH), and hypochlorous acid (HOCl), to overcome the defensive capacity of cumulus cells and elucidate the mechanism through which ROS differentially deteriorate oocyte quality.
Daniel J Jagger et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(48), 15851-15860 (2014-11-28)
The loss of auditory hair cells triggers repair responses within the population of nonsensory supporting cells. When hair cells are irreversibly lost from the mammalian cochlea, supporting cells expand to fill the resulting lesions in the sensory epithelium, an initial
Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123)
Axelsen LN, et al.
Journal of Molecular and Cellular Cardiology, 40(6), 790-798 (2006)
Xiaoting Hong et al.
Oncotarget, 6(17), 15566-15577 (2015-05-16)
The invasiveness of high-grade glioma is the primary reason for poor survival following treatment. Interaction between glioma cells and surrounding astrocytes are crucial to invasion. We investigated the role of gap junction mediated miRNA transfer in this context. By manipulating
Multimodal tumor suppression by miR-302 cluster in melanoma and colon cancer
Maadi H, et al.
The International Journal of Biochemistry & Cell Biology, 81, 121-132 (2016)
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