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C7901

Sigma-Aldrich

Chelex® 100 sodium form

50-100 mesh (dry)

Synonym(s):

Iminodiacetic Acid Resin

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About This Item

CAS Number:
11139-85-8
MDL number:
MFCD00163980
UNSPSC Code:
23151817
NACRES:
NA.56

form

beads

Quality Level

200

matrix

1% cross-linked polystyrene
 cross-linked polystyrene beads

matrix active group

iminodiacetic acid (sodium form)
paired iminodiacetate groups

matrix attachment

through methyl group to aromatic rings

particle size

50-100 mesh (dry)

pH

4-14

capacity

≥0.3 meq/mL binding capacity
 0.6 meq/g binding capacity (heavy metal ions)

Application

Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Chelex® 100 sodium form has been used:
  • for the extraction of DNA from bee samples
  • to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
  • to extract DNA from bacterial cultures

Biochem/physiol Actions

Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.

Other Notes

Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000414956
0000414956
0000410726
0000410726
0000409003

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What solvent should I use to swell the Product C7901, Chelex 100 at the beginning?

    We recommend using a good grade of water (deionized or distilled).

  4. My Chelex 100 (Product C7901) in my column dried out.  Can I still use it?

    The resin may be rehydrated and used, as long as it remains white.  If the color of the re-swollen resin is yellow, do not use it.

  5. I want to use Product C7901, Chelex 100, to make a metal-free solution.  However, the water wash from the Chelex resin is very basic.  How can I fix this?

    A suspension of Chelex 100 (sodium form) in water typically has a pH of 10-11. This pH can be slowly lowered by extended water washing, or by using a buffer.  The sodium form at pH 6.3 can be obtained by rinsing with at least 4 bed volumes of 0.5 N sodium acetate buffer, followed by 5 bed volumes of water. This is a more common method for making metal-free distilled water.

  6. What is the particle size of the resin in Product C7901, Chelex 100?

    The particle size for this resin is 45-165 microns. The mean particle size is 90 microns.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Elizabeth Perez et al.
Journal of immunology (Baltimore, Md. : 1950), 205(9), 2489-2498 (2020-09-23)
Chronic obstructive pulmonary disease (COPD) is a debilitating lung disease associated with cigarette smoking. Alterations in local lung and systemic iron regulation are associated with disease progression and pathogenesis. Hepcidin, an iron regulatory peptide hormone, is altered in subjects with
Methods for extracting genomic DNA from whole blood samples: current perspectives
Chacon Cortes D, et al.
Journal of Biosciences, 2014(2), 1-9 (2014)
Simultaneous detection of Escherichia coli O175: H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR
Germini A, et al.
Food Control, 20(8), 733-738 (2009)
Vit Molva et al.
Insect science, 27(2), 266-275 (2018-08-14)
The interaction of house dust mites (HDM) and microorganisms is the key factor in the survival of these mites in human-made environments. Spent growth medium (SPGM) provides the rest of the diet, along with dead mite bodies and microorganisms. SPGM
Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing
Sopko B, et al.
Journal of Apicultural Research, 59(4), 503-514 (2020)
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