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C7805
Anti-Collagen Type III (COL3A1) Antibody
mouse monoclonal, FH-7A
Synonym(s):
Anti-Collagen III antibody
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5 G
CN¥301.37
25 G
CN¥478.72
100 G
CN¥1,937.50
All Photos(3)
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5 G
CN¥301.37
25 G
CN¥478.72
100 G
CN¥1,937.50
About This Item
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Product Name
Monoclonal Anti-Collagen, Type III antibody produced in mouse, clone FH-7A, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
FH-7A, monoclonal
mol wt
antigen 70 kDa
contains
15 mM sodium azide
species reactivity
human, rat
1 of 4
This Item | 299693 | 638560 | 843951 |
|---|---|---|---|
| assay 98% | assay 98% | assay 97% | assay - |
| density 1.259 g/mL at 25 °C (lit.) | density 1.143 g/mL at 25 °C (lit.) | density 1.148 g/mL at 25 °C (lit.) | density 1.259 g/cm3 at 25 °C |
| form liquid | form liquid | form - | form liquid |
| refractive index n20/D 1.471 (lit.) | refractive index n20/D 1.467 (lit.) | refractive index n20/D 1.4470 (lit.) | refractive index - |
| bp 105-107 °C/2 mmHg (lit.) | bp 166 °C/18 mmHg (lit.) | bp 76 °C/0.8 mmHg (lit.) | bp - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
General description
Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X.
Monoclonal Anti-Collagen Type III (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen type III.
Specificity
Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X.
Immunogen
human collagen type III
Application
Monoclonal Anti-Collagen, Type III antibody produced in mouse has also been used in enzyme linked immunosorbent assay (ELISA) and immunohistochemistry.
Monoclonal anti-collagen type III antibody has been used in immunofluorescence and western blot.
Biochem/physiol Actions
This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Monoclonal antibodies have recently been developed against all of the major collagen types isolated from both human and other species. These antibodies have several potential advantages over polyclonal antibodies, and a brief survey will be made of the different antibodies
Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Krahn KN, et al.
Analytical Biochemistry, 350(2), 177-185 (2006)
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