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C7488

Sigma-Aldrich

Anti-CENP-E antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Centromere-associated protein-E

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About This Item

MDL number:
MFCD05665206
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~310 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line.
microarray: suitable
western blot: 1:2,000 using whole extract of HeLa nuclear cell line

UniProt accession no.

Q02224

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CENPE(1062)

Related Categories

General description

Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A.

Immunogen

synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH.

Application

Anti-CENP-E antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • immunocytochemistry.

Biochem/physiol Actions

Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint
Yao X, et al.
Nature Cell Biology, 2(8), 484-484 (2000)
CENP-E function at kinetochores is essential for chromosome alignment
Schaar BT, et al.
The Journal of Cell Biology, 139(6), 1373-1382 (1997)
Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure
Garcia SI, et al.
Acta Crystallographica Section D, Biological Crystallography, 60(6), 1158-1160 (2004)
Determination of the proteomic response to lapatinib treatment using a comprehensive and reproducible ion-current-based proteomics strategy
O Connell K, et al.
Journal of proteomics and genomics research, 1(3), 27-27 (2013)
CENP-E as an essential component of the mitotic checkpoint in vitro
Abrieu A, et al.
Cell, 102(6), 817-826 (2000)
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