C7104
Anti-Cdc27 antibody,Mouse monoclonal
clone AF3.1, purified from hybridoma cell culture
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
AF3.1, monoclonal
form
buffered aqueous solution
mol wt
antigen 97 kDa
species reactivity
mouse, Xenopus, rat, bovine, canine, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using a HeLa Cell nuclear extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)
Related Categories
General description
Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.
Immunogen
synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.
Application
Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
- Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
- Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
- Immunoprecipitation.
- Indirect ELISA
- Microarray
- Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
- Anaphase-promoting complex/cyclosome APC/C Purification.
Biochem/physiol Actions
CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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Sanjay Shete et al.
Cancer research, 71(24), 7568-7575 (2011-11-01)
Gliomas, which generally have a poor prognosis, are the most common primary malignant brain tumors in adults. Recent genome-wide association studies have shown that inherited susceptibility plays a role in the development of glioma. Although first-degree relatives of patients exhibit
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B
King R W, e al.
Cell, 81(2), 279-288 (1995)
Munemichi Ohe et al.
Molecular biology of the cell, 21(6), 905-913 (2010-01-22)
Emi2 (also called Erp1) inhibits the anaphase-promoting complex/cyclosome (APC/C) and thereby causes metaphase II arrest in unfertilized vertebrate eggs. Both the D-box and the zinc-binding region (ZBR) of Emi2 have been implicated in APC/C inhibition. However, it is not well
Po-Yuan Ke et al.
Molecular and cellular biology, 24(2), 514-526 (2004-01-01)
The expression of human thymidine kinase 1 (hTK1) is highly dependent on the growth states and cell cycle stages in mammalian cells. The amount of hTK1 is significantly increased in the cells during progression to the S and M phases
Liyong Zhang et al.
The Journal of biological chemistry, 286(12), 10041-10050 (2011-01-07)
Loss of TGF-β-induced growth inhibition is a hallmark of many human tumors. Previous studies implied that activation of the anaphase-promoting complex (APC/cyclosome) is involved in the TGF-β signaling pathway, which facilitates the destruction of SnoN, a transcriptional co-suppressor, which leads
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