C6870
Anti-COMT antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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Synonym(s):
Anti-Catechol-O-methyltransferase
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~24 kDa (and ~28 kDa)
species reactivity
human, mouse, rat
technique(s)
immunoprecipitation (IP): 5-10 μL using human HepG2 cell lysates
western blot: 1:250-1:500 using whole extracts of mouse and rat liver
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COMT(1312)
mouse ... Comt1(12846)
rat ... Comt(24267)
General description
Catechol-O-methyltransferase (COMT) is encoded by the gene mapped to human chromosome 22q11.21. It is widely expressed in neuronal and non-neuronal tissues. In mammals, two isoforms of COMT have been identified a cytosolic soluble form (S-COMT) and a membrane-bound form (MB-COMT), whose relative levels differ in various tissues and species.
Specificity
The antibody recognizes MB-COMT and S-COMT.
Application
Anti-COMT antibody produced in rabbit has been used in enzyme linked immunoassay (ELISA).
Anti-COMT antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μL using human HepG2 cell lysates and western blotting at a dilution of 1:250-1:500 using whole extracts of mouse and rat liver.
Biochem/physiol Actions
Catechol-O-methyltransferase (COMT) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamine neurotransmitters including dopamine, noradrenaline, and adrenaline, their metabolites, and L-DOPA. Its major physiological role is to inactivate biologically active or toxic catechols. Variations in enzymatic activity of COMT result in the pathogenesis of different psychiatric and neurological diseases. COMT inhibitors are important therapeutic agents used in the treatment of Parkinson′s disease.
Catechol-O-methyltransferase (COMT) is referred as HEL-S-98n. The COMT Met/Met genotype individuals can acquire skill in executive function tasks (as multi-UV supervisory control to a higher level and faster than other genotype groups). The genetic variants of COMT alter neurobehavioral functions that are affected by mercury in adults and might modify the adverse neurobehavioral effects of mercury exposure in children.
Target description
COMT (Catechol-O-methyltransferase) catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Weimin Kong et al.
British journal of pharmacology, 180(13), 1690-1709 (2023-01-25)
Liver failure is associated with psychiatric alterations, partly resulting from the increased brain dopamine levels. We investigated the relationship between increased dopamine levels and mental abnormalities using bile duct ligation (BDL) rats and the mechanism by which liver failure increased
Distribution of catechol-O-methyltransferase (COMT) proteins and enzymatic activities in wild-type and soluble COMT deficient mice
Myohanen T, et al.
Journal of Neurochemistry, 113(6), 1632-1643 (2010)
The chemistry of catechol-O-methyltransferase inhibitors
International Review of Neurobiology, 95(4), 119-162 (2010)
Haplotypic association spanning the 22q11. 21 genes COMT and ARVCF with schizophrenia
Sanders AR, et al.
Molecular Psychiatry, 10(4), 353-353 (2005)
Deficiency in catechol-o-methyltransferase is linked to a disruption of glucose homeostasis in mice.
Megumi Kanasaki et al.
Scientific reports, 7(1), 7927-7927 (2017-08-13)
2-methoxyestradiol (2-ME), an estrogen metabolite generated via catechol-o-methyltransferase (COMT), is multifunctional methoxy-catechol. Here, we report that COMT deficiency leads to glucose intolerance and 2-ME rescues COMT-deficient-associated metabolic defects. Liver COMT protein was suppressed in high fat diet (HFD)-fed or in
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