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C6417

Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 7 antibody produced in mouse

clone LDS-68, ascites fluid

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MDL number:
MFCD00162253
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LDS-68, monoclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human and animal tissue
microarray: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P08729

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KRT7(3855)

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General description

Keratin, type II cytoskeletal 7 is a protein encoded by the KRT7 gene in humans. It is also known as cytokeratin-7 (CK-7), keratin-7 (K7), sarcolectin (SCL) and K2C7. Keratin 7 is a type II keratin and is a member of the keratin gene family. It is found on chromosome 12. It is specifically expressed in the simple epithelia lining of the internal organs and in the gland ducts as well as blood vessels.
Monoclonal anti-Cytokeratin Peptide 7 (mouse IgG1 isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes of an immunized mouse. Cytokeratins are the intermediate filaments of endocrine cells. These proteins belongs to the intermediate filament (10 nm) superfamily of cytoskeletal proteins. Cytokeratin 7 (CK 7/ KRT7) is a low molecular weight cytokeratin. It is present in epithelial tissues of lung, breast and ovary.

Specificity

The antibody reacts specifically with most simple epithelia but shows no reaction with epithelial cells in the intestine or in the stomach.

Immunogen

cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Application

Monoclonal Anti-Cytokeratin Peptide 7 antibody produced in mouse has been used in:
  • immunohistochemical staining
  • western blotting
  • immunohistochemistry
  • immunofluorescence.

Biochem/physiol Actions

Cytokeratins may play an important role in transhepatic transport and canalicular secretion. Cytokeratin 7 (CK 7/ KRT7) only stains bile ducts in normal liver, hence it is considered as “bile duct keratin”. CK 7 is widely used in surgical pathology. It acts as a specific marker for mammary and extramammary Paget′s disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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